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fixable viability dye efluor 780  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fixable viability dye efluor 780
    Fixable Viability Dye Efluor 780, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fixable+viability+dye+efluor%C2%AE+780/PBS/bio_rxiv__2025__08__31__672068-227-13-18
    Average 99 stars, based on 1 article reviews
    fixable viability dye efluor 780 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Evaluation of adenoviral vector Ad19a encoding RSV-F as novel vaccine against respiratory syncytial virus
    Article Snippet: After stimulation, the surface staining was performed with anti-CD8a-Pacific blue (1:2000; 53-6.7, BD Biosciences), anti-CD4-PerCP (1:2000; RM4-5, Thermofisher), and Fixable Viablilty Dye eFluor® 780 (1:4000; eBioscience) in FACS-PBS for 20 min at 4 °C.

    Article Title: Age-related impairment of intestinal inflammation resolution through an eicosanoid-immune-microbiota axis.
    Article Snippet: For surface staining, cells were firstly stained with the Fixable Viability Dye eFluor® 780 (eBioscience) on ice for 30 min to exclude dead cells.

    Article Title: Tissue-resident neutrophils serve homeostatic and immunological functions in embryos
    Article Snippet: Prior to antibody staining, cells were stained with Fixable Viability Dye eFluor® 780 or 506 (65-0865-14 or 65-0866-14, respectively, Thermo Fisher) and Fc-receptor binding was blocked with anti-CD16/CD32 antibody (clone 2.4G2, BE0307, Bio X Cell).

    Article Title: Expanding cells in a bioreactor
    Article Snippet: To this end, the cells may be stained with the following antibody conjugates and gated against viable, unstained cells: Fixable Viability Dye eFluor® 780 (eBioscience 65-0865), mouse anti-human CD4-PE (BD Pharmingen 561844), anti-CD4-Alexa Fluor 647 (BD Pharmingen 557707), anti-CD4-FITC (BD Pharmingen 561842), anti-CD4-FITC (BD Pharmingen 555346), anti-CD25-PE (BD Pharmingen 555432), anti-CD127-PE (BD Pharmingen 557938), anti-CD45RO-PE (BD Pharmingen 347967), and anti-FoxP3-Alexa Fluor 647 (BD Pharmingen 560045).

    Article Title: A probiotic approach identifies a Treg-centred immunoregulation via modulation of gut microbiota metabolites in people with multiple sclerosis and healthy individuals.
    Article Snippet: Methods In this exploratory cohort study, people with MS (pwMS) and healthy controls (HC) supplemented a lactobacilli-rich probiotic for two or six weeks and were additionally investigated six weeks after the last intake.. Immune cell phenotyping was performed in blood samples, complemented by mRNA expression analysis, serum cytokine measurements, and Treg suppression assays.. Besides gut microbiota composition analysis, metabolite production was investigated in stool and serum.

    Article Title: Distinct tissue-niche localization and function of synovial tissue myeloid DC subsets in health, and in active and remission Rheumatoid Arthritis
    Article Snippet: All live cells or CD45 pos immune cells, were FACS sorted, after exclusion of dead cells with Fixable Viability Dye eFluor® 780 (eBiosciences).

    Article Title: Dietary galactose exacerbates autoimmune neuroinflammation via advanced glycation end product-mediated neurodegeneration
    Article Snippet: The cells were stained with the fixable viability dye eFluor ® 780 (0.2 μl/test, eBioscience) for 20 min. Nonspecific Fc-mediated interactions were blocked by addition of 0.5 μl anti-CD16/32 (93, eBioscience) for 10 min before the addition of αO4-APC (130-109-153, Milteniy Biotec) for 30 min.

    Binding Assay:

    Article Title: Evaluation of adenoviral vector Ad19a encoding RSV-F as novel vaccine against respiratory syncytial virus
    Article Snippet: After stimulation, the surface staining was performed with anti-CD8a-Pacific blue (1:2000; 53-6.7, BD Biosciences), anti-CD4-PerCP (1:2000; RM4-5, Thermofisher), and Fixable Viablilty Dye eFluor® 780 (1:4000; eBioscience) in FACS-PBS for 20 min at 4 °C.

    Article Title: Age-related impairment of intestinal inflammation resolution through an eicosanoid-immune-microbiota axis.
    Article Snippet: For surface staining, cells were firstly stained with the Fixable Viability Dye eFluor® 780 (eBioscience) on ice for 30 min to exclude dead cells.

    Article Title: Tissue-resident neutrophils serve homeostatic and immunological functions in embryos
    Article Snippet: Prior to antibody staining, cells were stained with Fixable Viability Dye eFluor® 780 or 506 (65-0865-14 or 65-0866-14, respectively, Thermo Fisher) and Fc-receptor binding was blocked with anti-CD16/CD32 antibody (clone 2.4G2, BE0307, Bio X Cell).

    Article Title: Expanding cells in a bioreactor
    Article Snippet: To this end, the cells may be stained with the following antibody conjugates and gated against viable, unstained cells: Fixable Viability Dye eFluor® 780 (eBioscience 65-0865), mouse anti-human CD4-PE (BD Pharmingen 561844), anti-CD4-Alexa Fluor 647 (BD Pharmingen 557707), anti-CD4-FITC (BD Pharmingen 561842), anti-CD4-FITC (BD Pharmingen 555346), anti-CD25-PE (BD Pharmingen 555432), anti-CD127-PE (BD Pharmingen 557938), anti-CD45RO-PE (BD Pharmingen 347967), and anti-FoxP3-Alexa Fluor 647 (BD Pharmingen 560045).

    Article Title: A probiotic approach identifies a Treg-centred immunoregulation via modulation of gut microbiota metabolites in people with multiple sclerosis and healthy individuals.
    Article Snippet: Methods In this exploratory cohort study, people with MS (pwMS) and healthy controls (HC) supplemented a lactobacilli-rich probiotic for two or six weeks and were additionally investigated six weeks after the last intake.. Immune cell phenotyping was performed in blood samples, complemented by mRNA expression analysis, serum cytokine measurements, and Treg suppression assays.. Besides gut microbiota composition analysis, metabolite production was investigated in stool and serum.

    Article Title: Distinct tissue-niche localization and function of synovial tissue myeloid DC subsets in health, and in active and remission Rheumatoid Arthritis
    Article Snippet: All live cells or CD45 pos immune cells, were FACS sorted, after exclusion of dead cells with Fixable Viability Dye eFluor® 780 (eBiosciences).

    Article Title: Dietary galactose exacerbates autoimmune neuroinflammation via advanced glycation end product-mediated neurodegeneration
    Article Snippet: The cells were stained with the fixable viability dye eFluor ® 780 (0.2 μl/test, eBioscience) for 20 min. Nonspecific Fc-mediated interactions were blocked by addition of 0.5 μl anti-CD16/32 (93, eBioscience) for 10 min before the addition of αO4-APC (130-109-153, Milteniy Biotec) for 30 min.

    Flow Cytometry:

    Article Title: Evaluation of adenoviral vector Ad19a encoding RSV-F as novel vaccine against respiratory syncytial virus
    Article Snippet: After stimulation, the surface staining was performed with anti-CD8a-Pacific blue (1:2000; 53-6.7, BD Biosciences), anti-CD4-PerCP (1:2000; RM4-5, Thermofisher), and Fixable Viablilty Dye eFluor® 780 (1:4000; eBioscience) in FACS-PBS for 20 min at 4 °C.

    Article Title: Age-related impairment of intestinal inflammation resolution through an eicosanoid-immune-microbiota axis.
    Article Snippet: For surface staining, cells were firstly stained with the Fixable Viability Dye eFluor® 780 (eBioscience) on ice for 30 min to exclude dead cells.

    Article Title: Tissue-resident neutrophils serve homeostatic and immunological functions in embryos
    Article Snippet: Prior to antibody staining, cells were stained with Fixable Viability Dye eFluor® 780 or 506 (65-0865-14 or 65-0866-14, respectively, Thermo Fisher) and Fc-receptor binding was blocked with anti-CD16/CD32 antibody (clone 2.4G2, BE0307, Bio X Cell).

    Article Title: Expanding cells in a bioreactor
    Article Snippet: To this end, the cells may be stained with the following antibody conjugates and gated against viable, unstained cells: Fixable Viability Dye eFluor® 780 (eBioscience 65-0865), mouse anti-human CD4-PE (BD Pharmingen 561844), anti-CD4-Alexa Fluor 647 (BD Pharmingen 557707), anti-CD4-FITC (BD Pharmingen 561842), anti-CD4-FITC (BD Pharmingen 555346), anti-CD25-PE (BD Pharmingen 555432), anti-CD127-PE (BD Pharmingen 557938), anti-CD45RO-PE (BD Pharmingen 347967), and anti-FoxP3-Alexa Fluor 647 (BD Pharmingen 560045).

    Article Title: A probiotic approach identifies a Treg-centred immunoregulation via modulation of gut microbiota metabolites in people with multiple sclerosis and healthy individuals.
    Article Snippet: Methods In this exploratory cohort study, people with MS (pwMS) and healthy controls (HC) supplemented a lactobacilli-rich probiotic for two or six weeks and were additionally investigated six weeks after the last intake.. Immune cell phenotyping was performed in blood samples, complemented by mRNA expression analysis, serum cytokine measurements, and Treg suppression assays.. Besides gut microbiota composition analysis, metabolite production was investigated in stool and serum.

    Article Title: Distinct tissue-niche localization and function of synovial tissue myeloid DC subsets in health, and in active and remission Rheumatoid Arthritis
    Article Snippet: All live cells or CD45 pos immune cells, were FACS sorted, after exclusion of dead cells with Fixable Viability Dye eFluor® 780 (eBiosciences).

    Article Title: Dietary galactose exacerbates autoimmune neuroinflammation via advanced glycation end product-mediated neurodegeneration
    Article Snippet: The cells were stained with the fixable viability dye eFluor ® 780 (0.2 μl/test, eBioscience) for 20 min. Nonspecific Fc-mediated interactions were blocked by addition of 0.5 μl anti-CD16/32 (93, eBioscience) for 10 min before the addition of αO4-APC (130-109-153, Milteniy Biotec) for 30 min.

    FACS:

    Article Title: Evaluation of adenoviral vector Ad19a encoding RSV-F as novel vaccine against respiratory syncytial virus
    Article Snippet: After stimulation, the surface staining was performed with anti-CD8a-Pacific blue (1:2000; 53-6.7, BD Biosciences), anti-CD4-PerCP (1:2000; RM4-5, Thermofisher), and Fixable Viablilty Dye eFluor® 780 (1:4000; eBioscience) in FACS-PBS for 20 min at 4 °C.

    Article Title: Age-related impairment of intestinal inflammation resolution through an eicosanoid-immune-microbiota axis.
    Article Snippet: For surface staining, cells were firstly stained with the Fixable Viability Dye eFluor® 780 (eBioscience) on ice for 30 min to exclude dead cells.

    Article Title: Tissue-resident neutrophils serve homeostatic and immunological functions in embryos
    Article Snippet: Prior to antibody staining, cells were stained with Fixable Viability Dye eFluor® 780 or 506 (65-0865-14 or 65-0866-14, respectively, Thermo Fisher) and Fc-receptor binding was blocked with anti-CD16/CD32 antibody (clone 2.4G2, BE0307, Bio X Cell).

    Article Title: Expanding cells in a bioreactor
    Article Snippet: To this end, the cells may be stained with the following antibody conjugates and gated against viable, unstained cells: Fixable Viability Dye eFluor® 780 (eBioscience 65-0865), mouse anti-human CD4-PE (BD Pharmingen 561844), anti-CD4-Alexa Fluor 647 (BD Pharmingen 557707), anti-CD4-FITC (BD Pharmingen 561842), anti-CD4-FITC (BD Pharmingen 555346), anti-CD25-PE (BD Pharmingen 555432), anti-CD127-PE (BD Pharmingen 557938), anti-CD45RO-PE (BD Pharmingen 347967), and anti-FoxP3-Alexa Fluor 647 (BD Pharmingen 560045).

    Article Title: A probiotic approach identifies a Treg-centred immunoregulation via modulation of gut microbiota metabolites in people with multiple sclerosis and healthy individuals.
    Article Snippet: Methods In this exploratory cohort study, people with MS (pwMS) and healthy controls (HC) supplemented a lactobacilli-rich probiotic for two or six weeks and were additionally investigated six weeks after the last intake.. Immune cell phenotyping was performed in blood samples, complemented by mRNA expression analysis, serum cytokine measurements, and Treg suppression assays.. Besides gut microbiota composition analysis, metabolite production was investigated in stool and serum.

    Article Title: Distinct tissue-niche localization and function of synovial tissue myeloid DC subsets in health, and in active and remission Rheumatoid Arthritis
    Article Snippet: All live cells or CD45 pos immune cells, were FACS sorted, after exclusion of dead cells with Fixable Viability Dye eFluor® 780 (eBiosciences).

    Article Title: Dietary galactose exacerbates autoimmune neuroinflammation via advanced glycation end product-mediated neurodegeneration
    Article Snippet: The cells were stained with the fixable viability dye eFluor ® 780 (0.2 μl/test, eBioscience) for 20 min. Nonspecific Fc-mediated interactions were blocked by addition of 0.5 μl anti-CD16/32 (93, eBioscience) for 10 min before the addition of αO4-APC (130-109-153, Milteniy Biotec) for 30 min.



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